Application

Immunoblotting (3-10 g/ml)

General description

Protein A purified rabbit polyclonal antibody. Recognizes the ~67 kDa GTAP protein.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin-protein ligases (E3s). GTAP is a member of the E2 ubiquitin-conjugating enzyme family. GTAP may participate in intercellular adhesion and embryonic development. GTAP is thought to play a role in stem cell development via its regulation of laminin-dependent embryonic cell adhesion and the formation of embryoid bodies.

Anti-GTAP, rabbit polyclonal, recognizes the ~67 kDa GTAP protein in HEK293 cells. It is validated for use in Western blotting.

Recognizes the ~67 kDa GTAP protein in HEK293 cells.Hu Entrez ID: 55585 Mu Entrez ID: 70093 Rat Entrez ID: 295292 Target NCBI Accession: HP 060052.3 Target SwissProt: Q7Z7E8

Immunogen

A synthetic peptide corresponding to amino acids 7-22 (QGQQQPGPGQQLGGQG) of human GTAP, conjugated to KLH.

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Due to a high degree of sequence identity, this antibody is also expected to react with mouse and rat GTAP. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Glass bottle

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)